Phagocytosis by osteoclasts

Biomaterial particle phagocytosis by bone-resorbing osteoclasts.

Abundant implant-derived biomaterial wear particles are generated in aseptic loosening and are deposited in periprosthetic tissues in which they are phagocytosed by mononuclear and multinucleated macrophage-like cells. It has been stated that the multinucleated cells which contain wear particles are not bone-resorbing osteoclasts. To investigate the validity of this claim we isolated human oste...

Cross-Presentation by Osteoclasts Induces

Nano-topography sensing by osteoclasts.

Bone resorption by osteoclasts depends on the assembly of a specialized, actin-rich adhesive 'sealing zone' that delimits the area designed for degradation. In this study, we show that the level of roughness of the underlying adhesive surface has a profound effect on the formation and stability of the sealing zone and the associated F-actin. As our primary model substrate, we use 'smooth' and '...

Phagocytosis by human monocytes.

By MARm J. CLINE AND ROBERT I. LEminn T HE BLOOD MONOCYTES of mammals are phagocytic cells which are almost certainly related to the phagocytic tissue macrophages of the reticuloendothelial system. Morphologic and cytochemical studies mdicate that mononuclear cells isolated from the peripheral blood or peritoneal cavity of mammals can differentiate, under appropriate circumstances, into large c...

Resorption of bone by isolated rabbit osteoclasts.

A carborundum wheel was used to prepare slices of cortical bone that demonstrate a predictable surface appearance in the scanning electron microscope. Osteoclasts were mechanically disaggregated from neonatal rabbit long bones and settled onto these slices. After 24h in culture osteoclasts were associated with areas of excavation in the bone surface. These excavated areas typically showed a wel...